Hemoglobin H Disease Classification by Isoelectric Focusing: Molecular Verification of 110 Cases from Thailand

Hemoglobin H disease classification by isoelectric focusing: molecular verification of 110 cases from Thailand.

or other substances in these presumably normal specimens. The Access PRL assay after PEG treatment may be suitable despite the CV of 14% because PEG treatment is frequently used to detect macroprolactin interference (6). It is possible that two samples with low recoveries contained macroprolactin. Our experience with this assay is that samples with PEG recovery values Ͻ60% contain high-molecula...

Pregnancies Complicated by Hemoglobin H disease

Dear Editor-in-Chief The recent report on “The Adverse Effects of Pregnancies Complicated by Hemoglobin H (HBH) Disease” is very interesting (1). Rabiee et al. reported a pregnant case complicated with HBH disease. Indeed, this problem might not common in the Middle East but it is very common in Southeast Asia. The authors hereby would like to share the experience on this top...

Quantification of hemoglobin variants by capillary isoelectric focusing.

Capillary isoelectric focusing (cIEF) was used to identify and quantify major and minor hemoglobin (Hb) variants. Whole blood (approximately 10 microL required) hemolysate was analyzed with a commercial instrument equipped with a 50 microns (i.d.) x 27 cm coated capillary filled with 20 g/L ampholytes (pH 6-8) in 4 g/L methylcellulose (MC). Cathode and anode solutions were 20 mol/L NaOH and 100...

Heterozygote Hemoglobin J Iran in Combination with Hemoglobin H Disease

This is a report concerning a concurrent case of hemoglobin J Iran (Hb J Iran) and Hemoglobin H (Hb H) disease in an Iranian woman. The patient was coincidentally found during the course of routine pre-marital genetic counselling for her son. The diagnosis of heterozygote Hb J Iran for her son, ultimately led to the diagnosis of concurrent Hb J Iran and Hb H disease. The hematological examinati...

Isoelectric focusing.